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390953
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A10663
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607445
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| Short Description | SDS-MW Analysis Kit | IgG Purity and Heterogeneity Kit | Wetting Tray - Retainer Version |
| Long Description | The SDS-Gel MW Analysis Kit is designed for use on the PA 800 Plus and is used for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID, Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • MW Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL | The IgG Purity and Heterogeneity Kit is designed for use on the PA 800 Plus and is used to assess the purity and heterogeneity of IgG molecules in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS. These products are separated using a replacable gel matrix within a capillary for separation. This kit will detect impurities as low as 0.1% and includes a standard, IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL | Tray used to keep the capillary ends immersed in deionized water on the GeXP instrument. |
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